Now after each cycle the temperature decreases by 1☌ hence for 15 cycles the temperature will decrease to 15☌ from the original temperature. Our annealing temperature is 55☌ so, select the temperature of 66☌ for the first round of amplification. In the first stage, decrease the annealing temperature by 1☌ after each cycle. Suppose the annealing temperature for our PCR is 55☌. Now, let us understand the process by taking one simple example.
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